Full Length Research Article
RETRACTED ARTICLE: Molecular Analysis of Cold Responsive (COR) Genes in Selected Sugarcane and Saccharum spontaneum L.
Shafee Ur Rehman1, Khushi Muhammad1, Hassan Sher2, Youxiong Que3, Rahmat ali1, Shahid Ali4, Ishtiaq Hassan1, Murad Ali Rahat4*
Adv. life sci., vol. 9, no. 4, pp. 547-551, December 2022
*– Corresponding Author: Murad Ali Rahat (Email: rahatmurad32@yahoo.com)
Authors' Affiliations
2. Centre for Plant Science and Biodiversity, University of Swat, Swat- Pakistan
3. Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou- China
4. Centre for Biotechnology and Microbiology, University of Swat, Swat- Pakistan
[Date Received: 22/08/2022; Date Revised: 24/10/2022; Date Published: 31/12/2022]
Important Note: This article was retracted on 20 July 2025.
Abstract
Introduction
Methods
Results
Discussion
References
Abstract
Background: Sugarcane (Saccharum derived) is an important commercially harvested crop in all parts of the world including tropical and subtropical areas. Saccharum hybrid is the tall perennial true grasses with sweet stalk rich in sucrose and it is the main source of sugar.
Methods: Initially, 23 genes differentially expressed during cold stress in other Andropogoneae tribe members were retrieved from NCBI GenBank and were investigated in the genome of selected sugarcane and Saccharum spontaneum L. Samples. Their presence in our samples was analyzed and confirmed through PCR and Agarose Gel Electrophoresis (AGE).
Results: Most of these (COR) genes (21/23) were confirmed in cold tolerant cultivars namely, SPSG-394, CP-851491 and Saccharum spontaneum L. while the least number of genes was observed in cold sensitive cultivar namely, CP-77400. Moreover 10 cold responsive genes, namely CBF1, CBF2, CBF3, COR 6.6, COR 78, COR 47, WCOR 80, WCOR14, C17 and 85KDA were sent for sequencing. Nucleotide sequences analysis of selected genes revealed the homology to stress responsive protein. Furthermore, during a conserved domain search, three conserved domains had been detected, namely gypsy transposon, zinc binding for reverse transcriptase and pepsin like aspartate proteases.
Conclusion: The analysis of cold responsive genes in sugarcane could help breeders to select cold tolerant sugarcane cultivars through PCR amplification.
