In-vitro study on antioxidant status of Zinc NPs and crude extract of Ayurvedic herbal formulation

Jaafar Faez Kadhim Alsadooni, Sura Razzaq Khudhair

Abstract


Background: To create and evaluate cutting-edge therapeutic composition on biosynthesized nanoparticles exhibiting varied biological activity, nanotechnology is a novel and forward-thinking method. Increased antioxidant activity decreased toxicity to free radicals as well as cancer cells, and other benefits are achieved carefully by regulating the form and nanoparticle dimensions.

Methods: Nanostructures biosynthesized utilizing Liv-Pro-08 AHF showed a strong antioxidant effect. The creation of novel and more efficient antioxidants may benefit from the usage of nanoparticles. It is noteworthy that the resulting nanoparticles exhibit more biological processes than the extract. This research aims to calculate if there are any noticeable differences in the behaviour of leaf extracts and the many nanomaterials obtained from the aqueous-based separation of Liv-Pro-08 Ayurveda herbal-based formulations.

Results: Several in-vitro free radical scavenging experiments were used to determine whether or not Liv-pro-08 nanoparticles possessed any antioxidant properties. Zinc nanoparticles generated from Liv-pro-08 ayurvedic formulation demonstrated dose-dependent suppression of DPPH, as well as concentration-dependent lowering power potential, indicating that the nanomaterials contain free radical scavenging capability to produce the antioxidant effect.

Conclusion: Nanoparticles might be useful for the development of newer and more potent antioxidants. It is worth mentioning that the resultant nanoparticles possess an elevated biological activity in comparison to the extract. The data represented in our study contribute to a novel and unexplored area of nano materials as alternative medicine.

Keywords: Antioxidants; Biosynthesis; Leaf extract; Liv-pro-08; Zinc   


Full Text:

PDF

References


Nasrollahzadeh M, Sajadi SM, Sajjadi M, Issaabadi Z (2019) An introduction to nanotechnology. Interface science and technology: Elsevier. pp. 1-27.

Feitosa VA, Pinto TdJA, Dua K, Cerize NNP (2021) Advances in polymeric nanoparticles for drug delivery systems in cancer: Production and characterization. Advanced Drug Delivery Systems in the Management of Cancer: Elsevier. pp. 331-341.

Akhtar MS, Panwar J, Yun Y-S. Biogenic synthesis of metallic nanoparticles by plant extracts. ACS Sustainable Chemistry & Engineering, (2013); 1(6): 591-602.

Sharpe E, Farragher-Gnadt AP, Igbanugo M, Huber T, Michelotti JC, et al. Comparison of antioxidant activity and extraction techniques for commercially and laboratory prepared extracts from six mushroom species. Journal of agriculture and food research, (2021); 4100130.

Sunil K, Suma A, Ashika B, Roy CL, Naresh S, et al. GCMS And FTIR analysis on the methanolic extract of coriandrum sativum leaves. European journal of pharmaceutical and medical research, (2018); 5455-457.

Hutchison JE. Greener nanoscience: a proactive approach to advancing applications and reducing implications of nanotechnology. ACS nano, (2008); 2(3): 395-402.

Liu J, Jia L, Kan J, Jin C-h. In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus). Food and chemical toxicology, (2013); 51310-316.

Mondal A, Chowdhury S, Mondal N, Shaikh W, Debnath P, et al. Insecticidal and fungicidal performance of bio-fabricated silver and gold nanoparticles. International Journal of Environmental Science and Technology, (2022); 1-20.

Angulo P. Nonalcoholic fatty liver disease. New England Journal of Medicine, (2002); 346(16): 1221-1231.

Austin LA, Mackey MA, Dreaden EC, El-Sayed MA. The optical, photothermal, and facile surface chemical properties of gold and silver nanoparticles in biodiagnostics, therapy, and drug delivery. Archives of toxicology, (2014); 881391-1417.

Aliyu AB, Ibrahim MA, Musa AM, Musa AO, Kiplimo JJ, et al. Free radical scavenging and total antioxidant capacity of root extracts of Anchomanes difformis Engl.(Araceae). Acta Pol Pharm, (2013); 70(1): 115-121.

Ansari MA, Khan HM, Alzohairy MA, Jalal M, Ali SG, et al. Green synthesis of Al 2 O 3 nanoparticles and their bactericidal potential against clinical isolates of multi-drug resistant Pseudomonas aeruginosa. World Journal of Microbiology and Biotechnology, (2015); 31153-164.

He L-H, Yao D-H, Wang L-Y, Zhang L, Bai X-L. Gut microbiome-mediated alteration of immunity, inflammation, and metabolism involved in the regulation of non-alcoholic fatty liver disease. Frontiers in microbiology, (2021); 12761836.

Arome D, Chinedu E. The importance of toxicity testing. Journal of Pharmaceutical and BioSciences, (2013); 4146-148.

Song JY, Kim BS. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess and biosystems engineering, (2009); 3279-84.

Arulselvan P, Fard MT, Tan WS, Gothai S, Fakurazi S, et al. Role of antioxidants and natural products in inflammation. Oxidative medicine and cellular longevity, (2016); 2016.

El-Maddawy ZK, El-Sawy AE-SF, Ashoura NR, Aboelenin SM, Soliman MM, et al. Use of zinc oxide nanoparticles as anticoccidial agents in broiler chickens along with its impact on growth performance, antioxidant status, and hematobiochemical profile. Life, (2022); 12(1): 74.

Suma A, Ashika B, Roy CL, Naresh S, Sunil K, et al. GCMS and FTIR analysis on the methanolic extract of red Vitis Vinifera seed. World Journal of Pharmaceutical sciences, (2018); 106-113.

Singh M, Manikandan S, Kumaraguru A. Nanoparticles: a new technology with wide applications. Research Journal of Nanoscience and Nanotechnology, (2011); 1(1): 1-11.

Virkutyte J, Varma RS. Green synthesis of metal nanoparticles: biodegradable polymers and enzymes in stabilization and surface functionalization. Chemical Science, (2011); 2(5): 837-846.

Al-Habori M, Al-Aghbari A, Al-Mamary M, Baker M. Toxicological evaluation of Catha edulis leaves: a long term feeding experiment in animals. Journal of ethnopharmacology, (2002); 83(3): 209-217.

Arora A, Sairam R, Srivastava G. Oxidative stress and antioxidative system in plants. Current science, (2002); 1227-1238.




DOI: http://dx.doi.org/10.62940/als.v11i2.1931

Refbacks

  • There are currently no refbacks.