Correlation between Neutrophil Gelatinase Associated with Lipocaline and Megalin in Type 2 Diabetic patients

Duaa Abdul-Jabbar Dawood, Emad Mohamed Rasheed, Ahmad Saadi Hassan

Abstract


Background: Type 2 Diabetes Mellitus (T2DM) is a metabolic disorder defined via elevated blood glucose caused by insufficiency of insulin-sensitive tissues to respond to insulin or defective insulin production by pancreatic β-cells due to environmental and genetic factors. Megalin is a glycoprotein that is mostly found in the proximal tubular cells, major role is to reabsorb albumin and other proteins that the glomerulus has filtered in the proximal tubules.

Methods: The purpose of research measure the concentration of megalin and Neutrophil Gelatinase Associated Lipocalin (NGAL) in type 2 patients, in the period from December 2022 to March 2023 at the Baghdad Teaching Hospital120 participants aged(30-60) years, the measured concentration of Megalin, NGAL, and fasting blood glucose in serum and whole blood estimated for glycated hemoglobin (HbA1C).

Results: The result indicates blood glucose concentration in patients with T2DM (344.975±63.68mg/dl) was significantly higher than control(106.62±12.59mg/dl), HbA1C (13.40±10.35%) in T2DM patients was significantly higher than control (4.310±0.336%), Megalin levels revealed for T2DM patients (220.70±42.47pg/ml) significantly higher than control (120.42±29.33pg/ml), NGAL(447.68±62.76pg/ml) in T2DM patients higher than control(264.93±51.79 pg/ml).

Conclusion: According to the results NGAL and Megalin levels were significantly higher in T2DM patients as compared to controls play a role in diabetes pathogenesis and as biomarkers for the early identification of diabetic nephropathy.


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References


Artasensi A, Pedretti A, Vistoli G, Fumagalli L. Type 2 diabetes mellitus: a review of multi-target drugs. Molecules, (2020); 25(8):1987.

Palermi S, Iacono O, Sirico F, Modestino M, Ruosi C, Spera R. The complex relationship between physical activity and diabetes: An overview. Journal of Basic and Clinical Physiology and Pharmacology, (2022); 33(5):535–47.

Chaudhary N, Tyagi N. Diabetes mellitus: An Overview. International Journal of Research and Development in Pharmacy & Life Sciences, (2018); 7(4):3030–3.

Andreadi A, Muscoli S, Tahmir R, Meloni M, Muscoli C, Ilari S. Recent Pharmacological Options in Type 2 Diabetes and Synergic Mechanism in Cardiovascular Disease. International Journal of Molecular Sciences, (2023); 24(2):1646.

Lodhi TI. Diabetes Mellitus in Older Women. Clinics in Geriatric Medicine, (2021); 37(4):491–507.

Wang S, Yuan T, Song S, Duo Y, Zhao T, Gao J. Medium-and Long-Term Effects of Dapagliflozin on Serum Uric Acid Level in Patients with Type 2 Diabetes: Journal of Personalized Medicine, (2022);13(1):21.

Bellia A, Iellamo F, De Carli E, Andreadi A, Padua E, Lombardo M. Exercise individualized by TRIMPi method reduces arterial stiffness in early onset type 2 diabetic patients: a randomized controlled trial with aerobic interval training. International Journal of Cardiology, (2017); 248:314–9.

Kukida M, Sawada H, Daugherty A, Lu HS. Megalin: a bridge connecting kidney, the renin-angiotensin system, and atherosclerosis. Pharmacological Research, (2020); 151:104537.

Nielsen ML, Mundt MC, Lildballe DL, Rasmussen M, Sunde L, Torres VE. Functional megalin is expressed in renal cysts in a mouse model of adult polycystic kidney disease. Clinical Kidney Journal, (2021);14(11):2420–7.

Saito A, Takeda T, Hama H, Oyama Y, Hosaka K, Tanuma A. Role of megalin, a proximal tubular endocytic receptor, in the pathogenesis of diabetic and metabolic syndrome‐related nephropathies: protein metabolic overload hypothesis. Nephrology, (2005);10:S26–31.

Kadhim SH, Ubaid MM, Alboaklah HKM. New biomarkers for diagnosing and treatment of kidney failure disease. kerbala journal of pharmaceutical sciences, (2022);1(20).

Abed IN, Al-timari US, Dwayyikh AT. Correlation between Neutrophil Gelatinase Associated with Lipocaline and Beta-2 Microglobulin with Other Renal Markers in Iraqi Patients with Multiple Myeloma. Journal of Techniques, (2022); 4(Special Issue):101–4.

El Omri N, Mekouar F, Assoufi N, Maaroufi A, Smaali J, Charef A. Evaluation of selected trace elements in the Moroccan type 2 diabetic patients.International Journal of Research in Medical Sciences, (2020); 8(1):78–81.

Bacos K, Gillberg L, Volkov P, Olsson AH, Hansen T, Pedersen O. Blood-based biomarkers of age-associated epigenetic changes in human islets associate with insulin secretion and diabetes. Nature Communications, (2016); 7(1):11089.

Nanayakkara N, Curtis AJ, Heritier S, Gadowski AM, Pavkov ME, Kenealy T. Impact of age at type 2 diabetes mellitus diagnosis on mortality and vascular complications: systematic review and meta-analyses. Diabetologia, (2021); 64:275–87.

Hu C, Lin L, Zhu Y, Zhang Y, Wang S, Zhang J. Association between age at diagnosis of type 2 diabetes and cardiovascular diseases: A nationwide, population-based, cohort study. Frontiers in Endocrinology, (2021);12:717069.

Hassan AS. Evaluation Iron (Fe) and lad (Pb) effect on Diabetes mellitus2 patients in Erbil Governorate. Al-Nisour Journal for Medical Sciences, (2022); 4(2).

Bahendeka S, Wesonga R, Mutungi G, Muwonge J, Neema S, Guwatudde D. Prevalence and correlates of diabetes mellitus in Uganda: a population‐based national survey. Tropical Medicine & International Health, (2016); 21(3):405–16.

Asiimwe D, Mauti GO, Kiconco R. Prevalence and risk factors associated with type 2 diabetes in elderly patients aged 45-80 years at Kanungu District. Journal of Diabetes Research, (2020); 2020:1–5.

Davidson KW, Barry MJ, Mangione CM, Cabana M, Caughey AB, Davis EM. Screening for prediabetes and type 2 diabetes: US Preventive Services Task Force recommendation statement. Journal of the American Medical Association, (2021); 326(8):736–43.

Ahmed AM, Khabour OF, Awadalla AH, Waggiallah HA. Serum trace elements in insulin-dependent and non-insulin-dependent diabetes: a comparative study. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, (2018); 887–92.

Dutta A, Dudhe AP, Deb S, Dasgupta S, Sarkar A. A study on assessment of HbA1c level as a diagnostic criterion of type 2 diabetes mellitus. Asian Journal of Medical Sciences, (2016); 7(1):49–52.

Akselrod D, Friger M, Biderman A. HbA1C variability among type 2 diabetic patients: a retrospective cohort study. Diabetology & Metabolic Syndrome, (2021); 13:1–7.

Bryniarski MA, Yee BM, Jaffri I, Chaves LD, Yu JA, Guan X. Increased megalin expression in early type 2 diabetes: role of insulin-signaling pathways. American Journal of Physiology, (2018); 315(5): 1191–207.

Marzolo M, Farfán P. New Insights into the Roles of Megalin / LRP2 and the Regulation of its Functional Expression. Biological Research, (2011); 89–105.

Romi M.M, Anggorowati N, Maulida DS, Wishka M , Suskalanggeng A. Upregulation of Megalin, Cubilin, NGAL mRNA expression in kidney may represent tubular injury and apoptosis in chronic condition of rat diabetic model. Medical Journal of Malaysia, (2021); 76(1): 87.

Ogasawara S, Hosojima M, Kaseda R, Kabasawa H, Yamamoto-Kabasawa K, Kurosawa H. Significance of urinary full-length and ectodomain forms of megalin in patients with type 2 diabetes. Diabetes Care, (2012); 35(5): 1112–8.

De S, Kuwahara S, Hosojima M, Ishikawa T, Kaseda R, Sarkar P. Exocytosis-mediated urinary full-length megalin excretion is linked with the pathogenesis of diabetic nephropathy. Diabetes, (2017); 66(5): 1391–404.

Khdher NN, Ahmeid MS, Saadoon IH. Evaluation of Serum Neutrophil Gelatinase Associated Lipocalin Level as an Early Biomarkers for Type 2 Diabetic Nephropathy. Annals of the Romanian Society for Cell Biology, (2021); 25(6): 14028–42.

Firu SG, Streba CT, Firu D, Tache DE, Rogoveanu I. Neutrophil Gelatinase Associated Lipocalin (NGAL)–a biomarker of renal dysfunction in patients with liver cirrhosis: Do we have enough proof? Journal of Medicine and Life, (2015); 8(Spec Issue):15.

Thipsawat S. Early detection of diabetic nephropathy in patient with type 2 diabetes mellitus: a review of the literature. Diabetes & Vascular Disease Research, (2021); 18(6):14791641211058856.

DyabAllawi AA, Nada SZ, Turki KM. Neutrophil gelatinase associated lipocalin (NGAL) in early detection of nephropathy in type 2 diabetic Iraqi patients. Journal of the Faculty of Medicine, (2017); 59(1): 74–8.

Sueud T, Hadi NR, Abdulameer R, Jamil DA, Al-Aubaidy HA. Assessing urinary levels of IL-18, NGAL and albumin creatinine ratio in patients with diabetic nephropathy. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, (2019); 13(1): 564–8.




DOI: http://dx.doi.org/10.62940/als.v10i0.1990

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