Analysis of mRNA Expression for Injury-Age Estimation

Nusrat Saba, Saadia Noreen, Mubarak Ali Anjum, Saqib Ali, Muhammad Jawad, Allah Rakha

Abstract


Background: Determination of wound age is pivotal in forensic medical sciences, criminal and civil cases for the construction of crime scene and answering the questions like, time of infliction, manner of wound infliction, how long the person survives after infliction of wound and characterizing antemortem or postmortem wounds. The findings differ considerably among individuals due to the biological variations. Previous developed method in the injury-age determination is clinical, microscopic, enzymatic reaction at the wound margins, histological, and immunohistochemistry with the pitfalls associated with it.

Methods: This study is conducted on blunt injuries, particularly lacerated wound (type of wound inflicted by blunt weapon) to analyze the different expression pattern in injury-age up to 72hrs in total 21 individuals randomly grouped in different time intervals. To determine the time of injury, transcript abundance of mRNA of Fibronectin (FBN), IL1β, VEGFA, and GM-CSF was analyzed by real time polymerase chain reaction. 18S-rRNA was used as control marker.

Results: Percent knockdown (%KD) was calculated to determine the expression of mRNA for the determination of injury-age. IL1β and GM-CSF showed the predictive behavior for wound age up to 36hrs, Fibronectin (FBN) showed predictive behavior up to 12hrs while VEGFA showed prediction beyond 72hrs.

Conclusion: The detection of gradual decrease of mRNA of Fibronectin (FBN), IL1β, VEGFA, and GM-CSF may provide an estimation of wound-age.

Keywords: mRNA Expression; Injury-Age Estimation; Antemortem wounds; Postmortem wounds


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References


Palagummi S, Harbison S, Elliot D, Fleming R. A multiplex analysis of RNA expression during injury healing in human dermal injuries for injury-age estimation. Forensic Science International: Genetics Supplement Series, (2013); 4(1): e17-e18.

Bauer M. RNA in forensic science. Forensic Science International: Genetics, (2007); 1(1): 69-74.

Takamiya M, Biwasaka H, Saigusa K, Nakayashiki N, Aoki Y. Wound age estimation by simultaneous detection of 9 cytokines in human dermal wounds with a multiplex bead-based immunoassay: an estimative method using outsourced examinations. Legal Medicine, (2009); 11(4): 186-190.

Takamiya M, Fujita S, Saigusa K, Aoki Y. Simultaneous detections of 27 cytokines during cerebral wound healing by multiplexed bead-based immunoassay for wound age estimation. Journal of neurotrauma, (2007); 24(12): 1833-1844.

Saukko P, Knight B Knight's forensic pathology fourth edition. 2015; CRC press.

Oehmichen M. Vitality and time course of wounds. Forensic science international, (2004); 144(2-3): 221-231.

Clark RA. Fibronectin (FBN) matrix deposition and Fibronectin (FBN) receptor expression in healing and normal skin. Journal of Investigative

Dermatology, (1990); 94(6): s128-s134.

Grinnell F, Billingham RE, Burgess L. Distribution of Fibronectin (FBN) during wound healing in vivo. Journal of Investigative Dermatology, (1981); 76(3): 181-189.

Galiano RD, Tepper OM, Pelo CR, Bhatt KA, Callaghan M, et al. Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells. The American journal of pathology, (2004); 164(6): 1935-1947.

Takamiya M, Kumagai R, Nakayashiki N, Aoki Y. A study on mRNA expressions of Fibronectin (FBN) in dermal and cerebral wound healing for wound age estimation. Legal Medicine, (2006); 8(4): 214-219.

Shiomi A, Usui T. Pivotal roles of GM-CSF in autoimmunity and inflammation. Mediators of inflammation, (2015); 2015.

Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, et al. Primer3—new capabilities and interfaces. Nucleic acids research, (2012); 40(15): e115-e115.

Koressaar T, Remm M. Enhancements and modifications of primer design program Primer3. Bioinformatics, (2007); 23(10): 1289-1291.




DOI: http://dx.doi.org/10.62940/als.v7i1.765

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